B 241 Molecular and Cellular Biology Lab

This course covers principles and practice of basic molecular and cellular biology techniques; Introduction to skills in a Molecular and Cellular Biology lab; Plasmids: transformation, isolation and restriction digestion; protein production, purification and functional assay; Small-interfering RNAs (siRNA) and gene expression; Isolation of RNA and Analysis of gene expression by Polymerase Chain Reaction (PCR) and RT-qPCR; Immunofluorescence: following cellular movement of a shuttling RNA-binding protein before and after stress.