This course provides a comprehensive overview of bioimaging techniques including fundamental concepts and applications, which allow biology students to design imaging experiments for their own research. The course covers basic optics and spectroscopy, advanced fluorescence microscopy, basics of electron microscopy, and single-molecule imaging techniques. The course also introduces label-free optical imaging methods including Raman and infrared microscopy, Second and Third harmonic microscopy and holographic microscopy. Introduction to advanced methods for manipulation of single cells and single molecules (optical and magnetic tweezers), Atomic Force Microscopy and some references to advanced applications of electron microscopy for imaging bio-samples will be also provided.