This course provides a board and in depth overview of cutting edge imaging techniques used in life science researches: advanced application of both Transmission and Scanning Electron Microscopy (TEM and SEM) to biological materials investigations; fluorescence imaging and related techniques, including selective-plane illumination microscopy, super- resolution fluorescence imaging, tissue clearing techniques, and advanced fluorescent protein technologies; Coherent Raman microscopy; Near-field optical microscopy and recent advances in endoscopy; digital holographic microscopy. Through hands-on learning, the students will also learn: basics of correlative light and electron microscopy (CLEM), cryo-methods for bio-samples preparation, EM-based methods for the 3d reconstruction of cells and tissues, basic and advanced fluorescence microscopy techniques including confocal microscopy and stimulated emissions depletion (STED) microscopy for cellular imaging.