This course aims at deepening the understanding of the synergism among the contemporary structural biology high-resolution techniques, i.e. high-resolution multidimensional nuclear magnetic resonance (NMR) spectroscopy and single crystal X-ray crystallography and cryogenic electron microscopy (cryoEM). The course introduces students to the modern concepts of biomacromolecular dynamic structures, molecular mobility, thus their functioning and dynamic integrated structural biology. The contemporary high-resolution biophysical techniques used to study the biomacromolecular matter in the different forms, solutions, precipitates, aggregates, phase-separated entities, hydrogels, etc. will be widely discussed and analyzed in terms of current cutting-edge spectroscopic techniques and prominent examples from the literature and ongoing own studies at the university.