This course will start with an overview of the structural features of proteins and some other biological macromolecules, and discuss how these features have evolved to support specific functions. The course will then proceed to introduce the major methods used to determine 3D structures of proteins and other biomolecules, in particular X-ray crystallography, Nuclear Magnetic Resonance, cryo-Electron Microscopy, Small Angle X-ray Scattering and computational methods (Bioinformatics, 3D Homology Modelling). We will discuss the strengths and weaknesses of each method and to instruct students on how these methods are best used and combined.